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KMID : 0613820090190081177
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Journal of Life Science
2009 Volume.19 No. 8 p.1177 ~ p.1182
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Isolation and Characterization of Plant Pathogen that Cause Soft Rot Disease in Napa Cabbage
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Kwon Young-Hee
Yoo Ah-Young
Yu Jong-Earn
Kang Ho-Young
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Abstract
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In order to establish in vitro infection model for research of plant pathogen based on tissue softening disease in napa cabbage, eighty independent bacterial strains were isolated from the softened napa cabbage tissues. Eight bacterial isolates were primarily screened with the generation of reproducible tissue softening disease to fresh napa cabbages within 24～48 hours after inoculation. Through various microbiological, biochemical and morphological examinations, three Gram (-) isolates which harbor independent biological properties were finally chosen, and named as RB1, RB2 and RB6. Collective results obtained from API 20E test and analyses of VITEK 2 COMPACT and nucleotide sequences of 16S rRNA of each isolate proposed that isolates RB1 and RB2 are close to the Erwinia carotovora subsp. odorifera, and RB6 is close to the Erwinia carotovora subsp. carotovora. These isolates grew optimally at 30℃ with neutral pH culture condition. The isolates caused softening tissue disease with dose-dependent manner regardless of pre-surface damages of napa cabbage. Minimum dose to cause soft rot disease for RB1, RB2 or RB6 were 8.0×10? CFU/㎖, 10? CFU/㎖ or 4.7×10? CFU/㎖, respectively. These isolates caused tissue softening disease to eggplant, paprika and napa cabbage out of 14 different tested vegetables, indicating that these isolates damages specific plant tissues. The bacterial isolates obtained in this research and in vitro plant infection model will be adapted in the understanding of the mechanism of pathogenesis by plant pathogen.
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KEYWORD
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Bacterial plant pathogen, soft rot disease, cabbage, Erwinia
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